Welcome to Thunderdome

Didja freeze frame at 1:01? Well, nevermind... he's in there a lot at 1:50+

Punisher? Yeah, he was in the first trailer a lot too.

Punisher, Scorpion, Tarantula, Hulk

rumor is he's the baddie and it might be grey Hulk

, Tombstone, Boomerang, Jean Grey (Phoenix), and The Hand.

The big question marks are:
-Daredevil: If you look closely at some of the shots of Spidey and The Hand diving at one another, some of The Hand are clearly not jumping at him. It's not Hulk because that wouldn't be fair. Punisher is already front and center so why hide him? Thus, people think it's Daredevil who has already met Tom Holland's Spider-Man.
-Kingpin (rumored to be the overall villain in this new trilogy)
-Any other X-Men* or Avengers
-Venom: I think Venom comes later when they do Secret Wars.

* - It really looks like they're doing a storyline where Spidey goes into a metamorphosis. This happened in the comics and one of the central figures was Wolverine. Wolverine, along with Daredevil, also happens to be one of the most common opponents of The Hand. I highly, highly doubt it's him, and certainly not Hugh Jackman. Wolverine and Jean Grey is a whole thing and the age difference between the actors is too great. Still, Marvel has to know that putting their two biggest properties in the same film is money. Not to mention with Hulk being there, they could deliver the fight everyone truly wants to see on the big screen. Again, doubt it. I think we'll see that in Doomsday or Secret Wars.

Nerd

I embrace that label.